FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007963071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476023
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60541.271787287589045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28880.6066933740596567No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27760.5831650991653765No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23950.5031269497482265No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16220.3407398382010953No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15550.32666488804112404No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14470.30397690867878235No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14320.30082580043401264No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13750.28885158910388786No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13640.28654077639105674No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13370.2808687815504713No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12720.26721397915646933No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12220.256710285007237No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12100.2541893984114213No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11840.2487274774538205No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11770.247256960272928No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11720.24620659085800473No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11460.24074466990040397No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11280.23696334000668035No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9850.206922774739876No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9610.2018810015482445No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9450.19851981942049018No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9360.19662915447362836No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9260.1945284156437819No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9130.19179745516498153No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8490.1783527266539642No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT8430.17709228335605634No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG8420.17688220947307168No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7430.15608489505759177No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7050.14810208750417522No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6820.14327038819552837No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6560.13780846723792758No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6470.13591780229106576No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6360.13360698957823466No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6340.1331868418122654No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6330.13297676792928073No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6300.1323465462803268No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6170.12961558580152638No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5990.12583425590780278No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5960.12520403425884885No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5610.11785144835438624No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5450.1144902662266319No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5320.11175930574783151No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5000.10503694149232286No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4870.10230598101352245No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4850.10188583324755315No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC4770.100205242183676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)