FastQCFastQC Report
Wed 8 May 2024
EGAF00007963073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451206
Total Bases13.9 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT59541.3195746510463071No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57671.278130166708776No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25210.5587248396519551No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21940.4862523991258982No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14870.3295612203738425No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14350.3180365509323901No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13430.2976467511513588No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13430.2976467511513588No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12870.28523556867594846No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12490.27681369485334856No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12310.2728243862005381No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12290.27238112968355915No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11750.2604132037251278No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11680.25886180591570146No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11550.25598063855533837No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11500.254872497262891No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11180.24778039299122792No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11020.24423434085539641No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10990.24356945607992803No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10070.22317965629889674No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9510.21076847382348637No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8870.1965842652801603No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8830.19569775224620242No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG8430.18683262190662359No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8370.18550285235568675No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8310.18417308280474995No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7870.17442143943121324No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7420.16444816779918708No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7340.16267514173127132No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6920.15336675487471355No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6640.14716116363700837No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6520.1445016245351347No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6200.1374095202634717No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6110.13541486593706642No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5950.1318688138012349No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5860.1298741594748297No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5840.12943090295785073No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5680.12588485082201922No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5660.1254415943050403No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5610.12433345301259292No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5180.1148034378975457No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4960.10992761621077733No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4940.1094843596937984No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4880.10815459014286158No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4860.10771133362588263No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4830.10704644885041423No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4690.10394365323156164No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4620.10239225542213536No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC4590.10172737064666694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)