FastQCFastQC Report
Wed 8 May 2024
EGAF00007963074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479537
Total Bases21 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA26320.548862757201217No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26130.5449006020390502No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24730.5157057745283472No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17730.36973163697483197No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG15740.32823327501318983No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13890.2896543958026179No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13500.28152155099606496No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12770.2662985337940555No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12640.2635875855252045No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG12270.25587180968309015No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12210.25462060278977433No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG12110.25253525796758125No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG11670.2433597407499317No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA11410.23793784421222972No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG10800.225217240796852No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10640.22188068908134306No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG10200.2127051718636935No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10020.20895155118374598No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9940.2072832753259915No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG9840.20519793050379848No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG9720.20269551671716676No Hit
CAGACGTGGTTTGGTGGACGGTTTGTTAGTCTGGGTAGAAGTAG9190.19164318915954348No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9080.1893493098551311No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8830.1841359477996484No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8060.16807879266876175No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG8010.1670361202576652No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7820.16307396509549837No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7660.15973741337998945No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG7100.14805948237570823No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6910.1440973272135414No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG6620.13804982722918147No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6550.13659008585364632No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6360.1326279306914795No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6190.12908284449375126No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG6130.12783163760043542No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6020.12553775829602304No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG5970.1244950858849265No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5890.12282681002717204No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5770.12032439624054035No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA5660.11803051693612798No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT5460.11385982729174182No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5170.10781232730738191No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5100.10635258593184675No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT5040.10510137903853092No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5000.1042672411096537No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4980.10385017214521507No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC4820.10051362042970614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)