FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007963075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479537
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT62591.3052173242106448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28000.5838965502140606No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27190.5670052571542967No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24210.5048619814529431No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16360.34116241291078686No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15880.3311527577642601No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14570.3038347405935309No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14550.3034176716290922No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13970.2913226716603724No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13780.2873605164982056No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13260.2765167234228016No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12880.2685924130984679No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12850.26796680965180997No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12350.2575400855408446No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12270.25587180968309015No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG12030.2508669821098268No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12030.2508669821098268No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11630.24252560282105448No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11620.24231706833883515No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10120.21103689600593906No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG10030.20916008566596528No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10000.2085344822193074No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9570.19956749948387714No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9460.1972736201794648No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9380.19560534432171034No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG9100.1897663788195697No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8700.1814249995307974No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8200.17099827541983204No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8040.1676617237043231No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7890.16453370647103352No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7070.1474338789290503No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6890.1436802582491028No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6870.14326318928466417No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6820.14222051687356763No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6580.13721568930030426No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6570.13700715481808495No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6230.12991698242262847No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6100.12720603415377751No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6080.12678896518933888No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5990.1249121548493651No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5630.11740491348947006No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5380.11219155143398736No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5320.11094034454067153No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5230.10906353420069775No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5100.10635258593184675No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5090.10614405144962745No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4920.10259896525189924No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4900.1021818962874606No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4870.1015562928408027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)