FastQCFastQC Report
Wed 8 May 2024
EGAF00007963076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460839
Total Bases20.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25590.5552915443354404No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25440.5520366114847051No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24440.5303370591464698No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17160.3723643181241171No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14500.31464350890441134No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13140.2851321177244113No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12700.27558431469558786No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12500.27124440422794077No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12050.2614796056757349No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA11640.25258278921705846No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11610.2519318026469114No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11480.24911086084294082No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG11110.24108202647779375No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG10790.2341381697295585No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA10610.23023225030867614No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9690.2102686621574997No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9520.2065797382599997No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG9450.20506076959632322No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9280.20137184569882322No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG9230.20028686808191148No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9000.19529597104411736No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG8850.19204103819338209No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8080.17533238289294092No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG8080.17533238289294092No Hit
CAGACGTGGTTTGGTGGACGGTTTGTTAGTCTGGGTAGAAGTAG8010.17381341422926447No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7740.16795453509794092No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7180.15580278578852919No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7140.15493480369499976No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6780.1471229648532351No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG6600.14321704543235272No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG6340.13757516182441157No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6290.1364901842074998No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6170.13388623792691157No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6140.1332352513567645No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5760.12498942146823512No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG5730.12433843489808806No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG5710.12390444385132335No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5610.12173448861749982No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5520.11978152890705865No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5500.11934753786029394No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT5120.11110170797176454No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC4880.10589381541058808No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT4810.10437484674691161No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4780.10372386017676455No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG4770.1035068646533822No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA4630.10046892732602927No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4620.10025193180264692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)