FastQCFastQC Report
Wed 8 May 2024
EGAF00007963077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460839
Total Bases14.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60581.3145588806502921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56491.22580771158691No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26200.5685282712617639No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22100.47956010667499926No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15660.3398149896167642No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14580.31637947309147013No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14080.3055296969223525No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13720.29771785808058776No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13400.29077400133235254No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13070.28361314906073487No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12730.2762353012657349No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12610.2736313549851467No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12040.2612626101523525No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12030.2610456146289702No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11880.25779068177823494No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11520.24997884293647024No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11280.24477095037529376No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11080.2404310399076467No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10870.23587413391661732No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9590.20809870692367616No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9340.20267381883911734No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9300.2018058367455879No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8990.195078975520735No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8440.18314422173470563No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8400.1822762396411762No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG7930.17207745004220562No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7830.1699074948083821No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7700.16708655300441153No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7450.1616616649198527No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7080.15363283055470567No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7050.1529818439845586No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6770.14690596932985273No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6300.13670717973088214No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6250.13562220211397039No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6210.134754220020441No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6060.1314992871697057No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5980.12976332298264687No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5890.12781036327220568No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5820.12629139460852923No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5620.12195148414088218No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5360.11630960053294101No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5120.11110170797176454No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5080.11023372587823513No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4930.10697879302749985No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG4910.10654480198073514No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4830.10480883779367632No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT4800.10415785122352926No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4780.10372386017676455No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4710.10220489151308808No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4670.10133690941955867No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC4610.10003493627926455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)