FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007963079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497583
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT32150.6461233603238052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30080.604522260607778No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17910.35993994971693166No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17600.3537098333343382No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG13780.27693872178108975No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC13650.2743260923303248No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12320.24759688333403673No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12210.24538619687569713No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG12190.24498425388327175No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11680.23473470757642442No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11510.23131819214080868No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10380.20860841306877448No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10330.207603555587711No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10040.20177538219754293No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9850.19795692376950177No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT9810.19715303778465101No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9560.1921287503793337No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9410.1891141779361433No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9360.18810932045507986No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8950.17986948911035947No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8760.17605103068231834No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA8760.17605103068231834No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8680.1744432587126168No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG8430.16941897130729947No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8250.16580148437547101No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA8170.1641937124057695No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8130.16338982642091873No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7830.15736068153453794No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7770.1561548525572618No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7660.15394416609892217No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7620.1531402801140714No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7550.1517334796405826No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7550.1517334796405826No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7420.14912085018981758No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT7350.14771404971632873No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7310.14691016373147797No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6610.13284215899658952No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6450.12962661505718642No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA6300.12661204261399606No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5410.10872557945106646No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5110.10269643456468569No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5080.1020935200760476No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5080.1020935200760476No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5050.10149060558740954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)