FastQCFastQC Report
Wed 8 May 2024
EGAF00007963081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471471
Total Bases14.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58741.2458878700916918No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT31840.6753331594095926No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16790.35611946439971914No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16370.3472111752366529No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG13430.2848531510951893No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC13310.28230792562002754No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11650.2470989732136229No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11640.24688687109069274No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11370.24116011377157873No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11220.2379785819276265No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG10560.22397984181423675No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT10120.21464734840531016No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9910.21019320382377704No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9560.20276962952122188No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9540.2023454252753616No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9240.19598236158745713No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT9200.19513395309573653No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9190.1949218509728064No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9150.1940734424810858No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8730.18516515331801958No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8650.18346833633457837No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8520.18071100873648646No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA8400.17816578326132468No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8200.1739237408027217No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8080.1713785153275599No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA7960.16883328985239815No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7620.1616218176727731No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7500.1590765921976113No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG7440.1578039794600304No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7120.15101671152626567No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7110.1508046094033355No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7080.15016830303454506No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7080.15016830303454506No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7020.14889569029696417No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6380.13532115442943468No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6330.13426064381478395No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6260.1327759289542729No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA6230.13213962258548245No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6050.1283217843727398No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5000.10605106146507419No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4900.10393004023577272No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG4870.10329373386698228No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG4780.10138481476061094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)