FastQCFastQC Report
Wed 8 May 2024
EGAF00007963083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499750
Total Bases15.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT33680.6739369684842421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29490.5900950475237619No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG17540.35097548774387194No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17160.3433716858429215No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC13640.27293646823411705No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG13530.27073536768384193No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG12650.25312656328164085No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12480.24972486243121558No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12460.2493246623311656No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11860.2373186593296648No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11790.23591795897948972No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10780.21570785392696348No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10680.21370685342671333No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT10650.21310655327663833No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10480.2097048524262131No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10190.20390195097548777No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT9740.19489744872436218No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9480.18969484742371187No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC9360.1872936468234117No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9230.18469234617308655No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9140.18289144572286145No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8970.1794897448724362No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8940.17888944472236118No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA8880.1776888444222111No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8540.1708854427213607No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA8430.16868434217108555No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG8330.16668334167083543No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8060.1612806403201601No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7940.15887943971985993No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7900.15807903951975988No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7870.15747873936968484No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7850.15707853926963483No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7800.15607803901950976No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7770.15547773886943472No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7350.1470735367683842No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7100.14207103551775888No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6790.1358679339669835No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6580.13166583291645823No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA6010.12026013006503251No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5790.11585792896448223No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5300.10605302651325664No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5120.10245122561280641No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5120.10245122561280641No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5010.10025012506253127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)