FastQCFastQC Report
Wed 8 May 2024
EGAF00007963085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480788
Total Bases14.9 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58891.224864181302362No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT31840.6622461459104637No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16620.3456825045550222No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15960.33195504047521984No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC13110.27267735467607346No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG12150.25271013419636096No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11980.2491742722364119No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11790.24522242651646878No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11430.23773471883657663No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11340.23586279191660356No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10790.2244232385167683No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10630.22109536843681624No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT9710.20196011547709175No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9580.1992562210371307No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9430.19613634283717563No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT9410.19572035907718163No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9290.19322445651721756No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9030.18781666763729543No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA8950.1861527325973194No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC8910.1853207650773314No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8830.18365683003735536No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8760.18220088687737632No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8650.17991297619740926No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8210.17076133347754102No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA7990.16618551211760693No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG7820.16264965015765784No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7780.16181768263766982No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7650.15911378819770874No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7620.15848981255771774No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7540.1568258775177417No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7180.14933816983784953No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7080.14725825103787948No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6990.14538632411790645No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6820.14185046215795735No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6790.14122648651796635No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6750.1403945189979783No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA6320.1314508681581071No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6310.1312428762781101No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6060.12604307927818498No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5290.1100277045184156No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5200.10815577759844255No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5080.10565987503847851No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4900.10191602119853241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)