FastQCFastQC Report
Wed 8 May 2024
EGAF00007963171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465504
Total Bases14.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61611.3235117206296831No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27270.5858166632295319No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26650.5724977658623771No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG20040.43050113425448544No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC19030.40880422080153983No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17210.36970681240118236No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16600.3566027359593043No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG16270.3495136454251736No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16070.34521722691963974No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG15860.3407059874888293No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15790.33920224101189245No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC15490.3327576132535918No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14270.3065494603698357No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG14140.30375678834123876No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG13800.2964528768818313No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG13500.2900082491235306No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13170.28291915858939987No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12020.2582147521825806No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11280.24231800371210557No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11170.239954973534062No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC10970.23565855502852823No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC10430.22405822506358697No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10090.21675431360417954No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9570.2055836254897917No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9240.19849453495566094No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8900.19119062349625351No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG8700.18689420499071974No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG8540.1834570701862927No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8480.18216814463463257No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8280.17787172612909877No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG8220.17658280057743864No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8210.17636797965216194No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7270.15617481267615316No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6880.14779679659036227No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6870.14758197566508557No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6770.1454337664123187No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6630.14242627345844505No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5940.12760362961435348No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5810.1248109575857565No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5720.12287756925826632No Hit
GACGGTCCGTACGCACCGTTCCCGCCTCGCC5550.11922561352856259No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5400.11600329964941225No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5400.11600329964941225No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4980.10698082078779131No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC4930.10590671616140786No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4730.10161029765587407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)