FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007963173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496739
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30890.6218557431568691No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29890.6017244468423055No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29350.5908535468324412No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG22050.443895083736127No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC19790.39839835406521334No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18280.3680000966302223No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17820.35873970032552305No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17790.35813576143608616No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG17710.35652525773092103No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17650.35531737995204726No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG17330.3488753651313869No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC16840.33901102993725074No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG16000.32210074103301733No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15410.31022327620742485No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG14970.30136550582901683No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG14440.2906959187822981No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14090.2836499650722009No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13220.26613573727853057No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12710.2558687761581031No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC11820.23795192243814153No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11670.23493222799095703No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC11600.23352303724893758No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10830.21802193908672363No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10400.20936548167146124No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG9920.19970245944047074No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG9820.1976893298090144No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9690.19507226128812113No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9540.19205256684093658No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9530.19185125387779095No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8550.17212258348951864No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG8470.17051207978435354No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8230.16568056866885827No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7860.15823198903246977No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7190.14474402050171217No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7190.14474402050171217No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7150.1439387686491296No Hit
GACGGTCCGTACGCACCGTTCCCGCCTCGCC7000.1409190742019451No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6730.1354836241970129No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6290.12662585381860494No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6260.12602191492916803No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6220.1252166630765855No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5900.11877464825592514No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5820.11716414455076006No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5470.11011819084066281No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5370.10810506120920643No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5180.10428011490943936No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC5160.1038774889831481No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5120.10307223713056554No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC5090.10246829824112864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)