FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007963175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474666
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60581.2762658374520188No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27960.5890457711317011No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27100.5709277681569778No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG20210.42577306990599706No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC17050.35919994269654876No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16820.3543544302730762No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG16790.3537224069134928No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG16720.35224768574113163No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16610.34993026675599265No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16490.34740217331765916No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16340.3442420565197423No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG16240.34213531198779773No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC15430.3250706812790467No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15080.31769707541724074No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG14460.3046352593191844No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG13170.2774582548570995No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12820.2700846489952935No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12280.25870822852279285No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12130.255548111724876No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11300.23806213210973612No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC11300.23806213210973612No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC10390.21889075686904055No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10130.21341322108598468No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9720.20477556850501197No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG9440.19887668381556714No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9300.19592724147084475No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9240.194663194751678No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8970.18897498451542769No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8290.17464912169820462No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG8280.17443844724501018No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG8240.17359574943223235No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7580.1596912355213982No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7520.15842718880223147No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6800.1432586281722306No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6760.14241593035945274No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6760.14241593035945274No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6740.14199458145306385No Hit
GACGGTCCGTACGCACCGTTCCCGCCTCGCC6360.13398895223167448No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6230.13125018434014654No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6190.13040748652736872No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5950.12535129965070174No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG5480.1154496003505623No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5340.11250015800583989No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC5150.10849734339514522No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5120.10786532003556186No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC4890.10301980761208934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)