FastQCFastQC Report
Wed 8 May 2024
EGAF00007963212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502046
Total Bases22 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA30500.6075140524971816No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA27050.5387952498376642No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT26660.5310270373631102No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21060.4194834736259227No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA14620.2912083753281572No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12200.2430056209988726No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG11550.23005860020794905No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10880.21671320954653556No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9590.19101835289993346No Hit
AACAGTCTGAATTGCCGCATCCACAGTGGAAACCGGCGGTGGGG9440.18803057887125882No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9240.18404688016635926No Hit
GACGGTCTGGATAGCCGCGTCTACGGTGGACACCGGCGGCGGGG9200.18325014042537935No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8800.17528274301558025No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8610.17149822924592567No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG8370.1667177908000462No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8050.16034387287220692No Hit
CACAGTCTGAATTGCTGCATCAACAGTGGATACCGGCGGCGGCG8030.15994550300171698No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7710.1535715850738777No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7250.14440907805260872No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7090.1412221190886891No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC6570.13086450245595024No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT6440.12827509829776554No Hit
ACGGGTCACCAGGGTACCGGACGCTTTAGTGTACGCTTCGGCGT6380.12707998868629566No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5850.11652318711831187No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5830.11612481724782191No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC5610.1117427486724324No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5530.11014926919047259No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5390.1073606800970429No Hit
ACGGCGGGCAACACGACGACGACGACGTTTCATTTTTGCGTAGC5210.1037753512626333No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG5170.1029786115216534No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5030.10019002242822371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)