FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007963213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502046
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT72621.4464809997490269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29930.5961605111882178No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG26780.5334172565860499No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21310.4244630970070472No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC15200.3027611015723659No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14390.2866271218175227No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12590.25077383347342674No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12080.24061540177593288No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG11070.22049772331619016No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG9840.19599797628105792No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9760.19440449679909808No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9560.19042079809419854No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9420.18763220900076885No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9120.18165666094341953No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9100.18125829107292957No Hit
CGTGTCCGTGGCCACTACCGTGCCCCATGGG8810.1754819279508252No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8690.17309170872788548No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8580.17090067444019075No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8080.16094142767794187No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7670.15277484533289779No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG6720.13385227648462492No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6670.13285635180840003No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6480.12907183803874545No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6310.12568569413958083No Hit
TACCAGGTTGGCATCGACGGCTCTAACCTGG6310.12568569413958083No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6160.12269792011090618No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6090.12130362556419133No Hit
GGTGCGCACAAACGTGGCCGCACCGGCCGCA6020.11990933101747649No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5830.11612481724782191No Hit
ATGTCCAACATGGACATCGATGGTATTAACA5410.10775904996753284No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5150.10258024165116343No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5140.10238105671591846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)