FastQCFastQC Report
Wed 8 May 2024
EGAF00007963224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485169
Total Bases21.3 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23630.4870467816369141No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17460.35987460039697505No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15550.32050687492399554No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14240.2935059742069258No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12440.2564054999391965No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11620.23950417277278638No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10550.2174500019580806No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10380.21394606827723947No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9640.19869365107828404No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9550.19683862736489757No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8650.1782883902310329No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8440.17396000156646446No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7910.16303597303207748No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7440.15334862697328147No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6540.13479838983941678No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6510.1341800486016213No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC6100.12572938501841627No Hit
CGGTTCGCGCGGCTCACGTGGTACGCGCGGTTCACGCGGAACAC6000.12366824755909796No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5840.12037042762418869No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5470.11274421902471098No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5390.11109530905725634No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5350.110270854073529No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5310.10944639908980171No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5060.10429355544150595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)