FastQCFastQC Report
Wed 8 May 2024
EGAF00007963228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489140
Total Bases21.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23810.4867727031115836No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17240.3524553297624402No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15570.31831377519728504No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA14850.3035940630494337No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12970.2651592591078219No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12340.25227951097845197No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11340.23183546632865845No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10480.21425358792983604No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9680.1978983522100012No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9340.19094737702907144No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9120.18644968720611685No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8110.1658012021098254No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7620.15578362023142658No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7480.15292145398045548No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7230.14781044281800712No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6510.13309073067015578No Hit
TTCGCGGGCACGCTGCAGGATCGCGTGCTGTTGGTCCATCGCAC6360.13002412397268676No Hit
CGGTTCGCGCGGCTCACGTGGTACGCGCGGTTCACGCGGAACAC5820.11898433986179825No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5740.11734881628981478No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5620.11489553093183955No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5600.11448665003884369No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5490.11223780512736639No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5440.11121560289487673No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5370.10978451976939116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)