FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007963231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465461
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61201.314825517068025No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT32480.6978028234374094No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21160.4546030709339773No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19240.4133536429475294No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16740.35964345025684213No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13760.2956209005695429No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11130.23911777785893987No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10300.22128599388563167No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10070.21634465615808846No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10030.21548529307503744No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9800.21054395534749418No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8180.17573975048392884No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7810.16779064196570712No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7570.16263446346740112No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7480.1607008965305364No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7330.15747828496909516No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA6920.14866981336782242No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6800.14609172411866944No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6660.14308395332799095No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG6230.13384580018519276No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5870.12611153243773376No Hit
CCTACCGATGGCCCGCCGCACCGTGGTGGCG5600.12031083162713954No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5540.11902178700256305No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5430.1166585385241728No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5370.1153694938995963No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5240.11257656387968057No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4960.10656102229832361No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4790.10290872919535687No Hit
ATGGTTGTCCAGCACAACATGCAGGCTGCGA4760.10226420688306861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)