FastQCFastQC Report
Wed 8 May 2024
EGAF00007963336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459215
Total Bases20.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25480.5548599240007404No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC24950.5433184891608506No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21020.4577376610084601No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18720.4076521890617684No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC18370.400030486809011No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18170.39567522837886393No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13230.28810034515423055No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13150.2863582417821718No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12500.27220365188419365No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12350.2689372080615834No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9790.2131899001557005No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9450.20578596082445041No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9230.2009951765512886No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9190.20012412486525918No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA9150.1992530731792298No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8790.19141360800496499No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8760.19076031924044293No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8510.18531624620275908No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7420.16158008775845736No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7360.16027351022941325No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7330.1596202214648912No Hit
GGTGCCGTTTGCTTTGGCGATGTCGTTTACGGTATCACCCGGTT7030.15308733381967055No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6470.14089261021525865No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6470.14089261021525865No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6330.13784392931415568No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6300.13719064054963362No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6140.13370643380551595No Hit
GAAATCCTGCTTCAGTTCGTTCCATTCCTCGTCCGTGAAGGTGT6120.1332709079625012No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5850.12739130908180266No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5500.11976960682904521No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5430.11824526637849372No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5210.11345448210533192No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA4850.10561501693106715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)