FastQCFastQC Report
Wed 8 May 2024
EGAF00007963340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461318
Total Bases20.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26120.5662037900103616No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25430.5512466454809914No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20260.43917644661600025No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC19060.41316402134753033No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18470.400374578923866No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17340.37587954512939015No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13780.2987093501662627No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13560.29394040553371No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC13090.2837522056368925No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12870.27898326100433973No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9810.21265157656974146No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9430.20441430856805934No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9010.19530995972409487No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA8990.1948764193029537No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8940.1937925682501008No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8890.1927087171972479No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8820.1911913257232538No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8820.1911913257232538No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8580.18598884066955984No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8340.18078635561586584No Hit
GGTGCCGTTTGCTTTGGCGATGTCGTTTACGGTATCACCCGGTT7310.15845902392709585No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6920.15000498571484314No Hit
GAAATCCTGCTTCAGTTCGTTCCATTCCTCGTCCGTGAAGGTGT6500.14090063687087867No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6400.13873293476517282No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6350.13764908371231993No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6160.13353044971147884No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6130.1328801390797671No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5980.12962858592120838No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5900.1278944242366437No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT5720.12399256044637322No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5350.11597206265526167No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4970.10773479465357952No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC4860.10535032233730311No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA4740.10274907981045613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)