FastQCFastQC Report
Wed 31 May 2023
EGAF00007963435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448599
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA40180.8956774312916435No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32370.721579851939037No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19420.43290332791646885No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18880.4208658512390799No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT18600.41462419666561895No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18090.40325546869252943No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17730.3952304842409368No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15740.3508701535224109No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC15030.3350431008539921No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14120.31475772349024406No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11010.24543077447787445No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10860.2420870309563775No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9510.21199333926290517No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC8240.1836829774475645No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7580.16897050595297805No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7480.16674134360531342No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC7120.15871635915372082No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6940.1547038669279245No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6520.14534138506773311No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6410.14288930648530201No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6380.14222055778100262No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG6380.14222055778100262No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6140.13687056814660753No Hit
CTGCCGACCTTCTCCAACATTGGTGTAGGCC6080.13553307073800877No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC5940.13241224345127833No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5560.12394142653015276No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5310.11836852066099122No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5190.11569352584379368No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5190.11569352584379368No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5100.11368727973089551No Hit
TCCCCGAAACCGTCTTGTCACGACGTTATCA5080.1132414472613626No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5080.1132414472613626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4990.11123520114846444No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4990.11123520114846444No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4920.10967478750509921No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC4810.10722270892266812No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4620.10298730046210534No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4560.10164980305350658No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4540.10120397058397367No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4490.10008938941014134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)