FastQCFastQC Report
Wed 8 May 2024
EGAF00007963447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442257
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64441.4570713408719365No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29830.6744946942614815No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20760.46941032024365925No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19610.4434073400760192No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19520.4413723242368125No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17810.4027070232918869No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC15370.34753548276228524No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14610.3303509045645405No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13090.29598174816905104No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11850.2679437521622043No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10650.24081020763944946No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8420.19038703740133No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8170.18473421562575607No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6950.1571484453609553No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6900.1560178810058405No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6380.1442600117126467No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6200.14018998003423347No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6200.14018998003423347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5430.12277928896546578No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5310.12006593451319028No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5220.11803091867398367No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT4750.1074036137359047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)