FastQCFastQC Report
Sun 21 May 2023
EGAF00007963451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502856
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT67771.3477019265952879No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17010.3382678142450324No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13470.26786992697710676No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13140.2613074120623002No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12990.258324450737388No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11240.22352323528008017No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9840.19568226291423388No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8620.17142084413828215No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7990.1588924065736513No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7820.15551171707208425No Hit
GCTGCTTCTAGCGATGCACTGGAAGCCCTGG7490.14894920215727764No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7210.14338100768410839No Hit
ATGTCCAACATGGACATCGATGGTATTAACA7040.14000031818254133No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6930.1378128132109391No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6480.12886392923620282No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6370.1266764242646006No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6070.1207105016147764No Hit
CTGGACACCAACTCCGTTTATGAACCGTACT5960.11852299664317419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5840.1161366275832445No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5720.11375025852331483No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5540.11017070493342031No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5510.10957411266843788No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5210.10360819001861367No Hit
CCGAACGGTGGTACCCTGACTAACCTGCTGG5170.10281273366530379No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5160.10261386957697632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)