FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007963561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434774
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60491.3912975476914442No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24910.5729413442386159No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA24870.5720213260222553No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20010.4602391127344322No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19320.4443687985022104No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18300.4209083339850129No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15820.36386720457065047No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14570.33511663530937913No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13970.32131636206396885No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12400.285205647071812No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12380.28474563796363167No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12240.2815255742063693No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11520.26496524631187696No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11360.26128517344643426No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10690.24587486832239278No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10410.239434740807868No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10190.2343746406178842No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10110.23253460418516286No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10010.23023455864426115No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9930.2283945222115398No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9160.21068417154659663No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8620.19826392562572742No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8430.19389383909801414No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8420.19366383454392397No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8180.18814372524575987No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7530.17319342922989875No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7480.17204340645944788No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6830.1570931104435868No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6370.1465129009554389No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6200.142602823535906No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5900.1357026869132009No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5850.13455266414275002No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5630.1294925639527663No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5450.12535248197914317No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC5390.12397245465460216No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5330.12259242733006114No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5300.12190241366779062No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4820.11086219507146242No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA4770.10971217230101157No Hit
AAACTGTCCTCTGGCTACCGCATCAACCGTG4620.10626210398965899No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4530.10419206300284745No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4420.10166201290785558No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA4410.1014320083537654No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4370.10051199013740474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)