FastQCFastQC Report
Wed 8 May 2024
EGAF00007963565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441417
Total Bases13.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61501.3932404053310135No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25900.5867467723263943No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA25830.5851609702390257No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT20150.456484458006828No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19160.4340566856283288No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18050.408910395385769No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14990.3395881898522259No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14520.32894066155132223No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14320.3244097984445547No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13560.3071925186388381No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13220.2994900513573333No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12670.2870301778137226No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12030.27253141587206653No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11330.2566733949983802No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10790.24444006461010792No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10730.24308080567807763No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10600.24013574465867876No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10320.23379253630920424No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA10300.2333394499985275No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10160.2301678458237902No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9230.2090993323773212No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9040.20479501242589207No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9000.20388883980453856No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8970.20320921033852346No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8580.19437402728032677No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7300.16537650339701462No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7220.1635641581543076No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7160.16220489922227735No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7030.15925983820287848No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6480.14679996465926776No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6370.14430798995054564No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5910.13388700480498034No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5710.1293561416982128No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5410.12255984703806151No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5390.12210676072738477No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC5270.11938824286332425No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC4970.11259194820317296No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA4920.11145923242648108No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4900.11100614611580432No Hit
ATGCTGCGTCTGCCGACCGTGTTCCGCCAGA4840.10964688718377406No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4520.10239750621294604No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4460.10103824728091577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)