FastQCFastQC Report
Wed 8 May 2024
EGAF00007963571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508386
Total Bases15.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT56551.1123437702847836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32310.6355407111918896No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20890.41090824688327376No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18070.3554385840680113No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13140.2584650246072866No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12820.25217059478427806No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12270.24135204352598222No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12150.23899163234235404No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11510.22640277269633702No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11390.22404236151270887No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT11190.22010834287332853No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10940.21519081957410316No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10700.21046999720684675No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9730.19139000680585225No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT9660.19001310028206914No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9540.18765268909844093No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9510.1870625863025339No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9480.18647248350662687No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9150.17998135275164934No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8990.1768341378401451No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8380.16483538099003514No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT7830.15401682973173927No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT7770.15283662413992516No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC7410.1457553905890406No Hit
GACGCCGCGTCTTCTTCTTCCGCGGGTCAGT7180.14123126915375325No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7160.14083786728981523No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6620.13021601696348836No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6470.12726550298395314No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6450.1268721011200151No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6020.11841396104534743No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5780.11369313867809105No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5580.10975912003871074No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5240.10307128835176421No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC5160.10149768089601208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)