FastQCFastQC Report
Wed 8 May 2024
EGAF00007963596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483913
Total Bases21.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA34790.7189308822040326No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA30380.6277987985443665No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA17220.355849088575839No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14650.3027403686199792No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12680.26203057161101273No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12420.2566577050006923No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11900.24591197178005136No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC11780.24343218719067272No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11390.23537288727519204No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11290.23330640011737647No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10630.2196675848757938No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9930.20520217477108488No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG9860.20375563376061398No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT9800.20251574146592466No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9640.19920936201341977No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9610.1985894158660751No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9350.19321654925575465No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9320.19259660310840998No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG9170.18949687237168666No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8430.17420486740385152No Hit
GGCGCCAATCAGACGGTGACGTTTCTGGGTGCGTTTAGATGGGA8010.1655256213410262No Hit
CTGCAGCTCGCGAGCGCTGATGTAACCATCGCCATCTTCATCGA7820.1615992957411766No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6680.13804134214207925No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6070.12543577047940435No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG6030.12460917561627814No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5690.11758311927970524No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5650.11675652441657902No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5580.11530998340610811No Hit
GATGTCACCCAGTTCGGTCAGCACATCTTTTTCCGGGTTTTCGT5560.11489668597454501No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5340.11035041422735078No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG5290.109317170648443No Hit
CAGTGGGTCACCCAGGGACAGCTGGCTGTTCACCTCTTTACGCA5070.10477089890124879No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG5030.10394430403812256No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG4860.1004312758698361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)