FastQCFastQC Report
Wed 8 May 2024
EGAF00007963597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483913
Total Bases15 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT77561.6027674396017468No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27920.5769632144621037No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19720.40751126752122796No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19390.4006918599004367No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13670.28248879447338676No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG13540.27980236116822654No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13330.2754627381368138No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12780.2640970587688283No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12260.2533513255481874No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11240.23227315653846867No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10800.22318061304408024No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10660.22028753102313844No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10640.21987423359157537No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT10550.21801439514954135No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10110.2089218516551529No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9980.20623541834999268No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9770.20189579531857998No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9670.1998293081607644No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG9440.19507638769778865No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG8170.16883200079353108No Hit
TCCCTGAACGACTCTTACTTCGCGTATGGCG7780.1607727008780504No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6610.13659480113160838No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6480.13390836782644816No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6360.1314285832370695No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6340.13101528580550637No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6270.12956874479503547No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5970.1233692833215888No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA5890.12171609359533636No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5760.11902966029017613No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5710.11799641671126834No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5700.11778976799548678No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5440.11241690138516634No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC5420.11200360395360323No Hit
ATGGAATCTTCTGCCAAACGTAAAATGGATC5360.1107637116589139No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG5160.1066307373432828No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4990.10311770917499634No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4910.10146451944874389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)