FastQCFastQC Report
Wed 8 May 2024
EGAF00007963599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451957
Total Bases14 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT72121.595727027128687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64261.4218166772502694No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18200.40269317656325715No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17870.395391596988209No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12600.27878758377456264No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12400.2743623840321092No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG12310.2723710441480052No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11900.2632993846759758No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11730.25953796489489045No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10200.22568518686512212No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10050.2223662870582821No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10010.22148124710979142No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9780.21639226740597003No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9710.21484344749611137No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT9530.21086076772790335No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9280.2053292680498366No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8850.1958150886035618No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8650.19138988886110847No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG8420.1863009091572871No Hit
TCCCTGAACGACTCTTACTTCGCGTATGGCG7380.16328987049652954No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG6970.15421821102450012No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6440.14249143170699868No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6250.13828749195166798No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5910.13076465238949722No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5760.1274457525826572No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5720.12656071263416652No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA5540.12257803286595849No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5520.12213551289171315No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5360.11859535309775046No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC5290.11704653318789177No Hit
ATGGAATCTTCTGCCAAACGTAAAATGGATC5170.11439141334241974No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5150.1139488933681744No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5140.11372763338105174No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4920.10885991366435302No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG4670.1033284139862863No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4620.10222211405067295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)