FastQCFastQC Report
Wed 8 May 2024
EGAF00007963600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487701
Total Bases21.4 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA35080.7192931734812928No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA31170.6391211008384236No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA17300.3547255388034882No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15140.31043610736906424No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC12640.25917519135699946No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12420.2546642307479378No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12220.2505633574669726No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11750.24092630525670441No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11740.24072126159265617No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11720.24031117426455964No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10460.21447567259447897No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG10320.21160506129780338No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10240.2099647119854173No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9810.20114783443134215No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9640.19766209214252176No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT9630.1974570484784735No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9500.19479148084584613No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9380.19233095687726703No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG8920.18289894833104708No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8600.1763375510815028No Hit
CTGCAGCTCGCGAGCGCTGATGTAACCATCGCCATCTTCATCGA7840.16075423261383512No Hit
GGCGCCAATCAGACGGTGACGTTTCTGGGTGCGTTTAGATGGGA7380.1513222240676152No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6660.13655908025614055No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6000.12302619842895544No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5970.12241106743681067No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5840.1197454998041833No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5740.11769506316370072No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5640.11564462652321812No Hit
GATGTCACCCAGTTCGGTCAGCACATCTTTTTCCGGGTTTTCGT5500.1127740152265425No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG5420.11113366591415641No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5400.11072357858605991No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT5170.10600757431294995No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG5110.10477731232866039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)