FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007963601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487701
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT78231.6040565838495309No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29640.6077494202390399No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21050.4316169128215854No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19230.3942989659648022No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG14160.29034182829233485No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13300.27270807318418455No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13230.2712727675358468No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12570.2577398857086617No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12430.25486927441198604No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11630.2384657812881253No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10920.2239076811406989No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10770.22083202617997505No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10670.21878158953949245No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10540.21611602190686507No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT10520.21570593457876855No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG10220.20955462465732078No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10070.2064789696965969No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10050.20606888236850035No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG9000.1845392976434332No Hit
TCCCTGAACGACTCTTACTTCGCGTATGGCG7820.16034414528573862No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG7650.1568584029969182No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6970.14291543384163657No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6680.13696916758423708No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6540.13409855628756145No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6300.12917750835040323No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6200.12712707170992063No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA6190.12692202804587238No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6110.1252816787334863No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6070.12446150407729327No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5990.12282115476490718No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5950.12200098010871414No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC5930.12159089278061765No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5610.11502949553107333No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5290.10846809828152905No Hit
ATGGAATCTTCTGCCAAACGTAAAATGGATC5130.1051873996567569No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4950.10149661370388824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)