FastQCFastQC Report
Wed 8 May 2024
EGAF00007963602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459137
Total Bases20.2 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA32200.7013157292921285No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA29330.6388071534204388No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA16470.3587164615354459No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13760.2996926843186239No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11740.25569710130091894No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11680.2543903018053435No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA11530.251123303066405No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC11430.2489453039071127No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11060.2408867070177311No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10220.22259151407967556No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT10190.22193811433188787No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG9580.2086523194602047No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT9510.20712772004870006No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9320.2029895216460446No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG9290.20233612189825695No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT8960.19514872467259226No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8830.19231732576551225No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8730.19013932660621993No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG8720.1899215266902907No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8130.17707133165046599No Hit
CTGCAGCTCGCGAGCGCTGATGTAACCATCGCCATCTTCATCGA7200.15681593946904737No Hit
GGCGCCAATCAGACGGTGACGTTTCTGGGTGCGTTTAGATGGGA7000.1524599411504627No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6070.1322045489690441No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5750.12523495165930865No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG5640.12283915258408709No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5500.11978995376107784No Hit
GATGTCACCCAGTTCGGTCAGCACATCTTTTTCCGGGTTTTCGT5460.11891875409736091No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5390.11739415468585629No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5280.11499835561063475No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5160.11238475661948394No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG4990.10868215804868699No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG4750.10345496006638542No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT4720.10280156031859773No Hit
CAGTGGGTCACCCAGGGACAGCTGGCTGTTCACCTCTTTACGCA4610.10040576124337615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)