FastQCFastQC Report
Wed 8 May 2024
EGAF00007963603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459137
Total Bases14.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT74031.612372777624108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66691.4525076393320513No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18470.40227644472129237No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18000.3920398486726184No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12670.2759524934823375No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG12630.27508129381862056No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12500.27224989491154056No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12310.26811169650888517No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11630.25330130222569736No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10190.22193811433188787No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10170.22150251450002942No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9960.21692871626551552No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9770.21279051786286007No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9710.2114837183672847No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9620.20952351912392164No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT9410.20494972088940774No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG9260.20168272215046923No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG8920.19427752500887535No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG8380.1825163295486968No Hit
TCCCTGAACGACTCTTACTTCGCGTATGGCG7280.1585583387964812No Hit
TTCCCGGTGCTGGCGAACGAGGGCAAATCTG7060.15376674064603812No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6240.13590714753984104No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6060.13198674905311486No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6020.13111554938939793No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5990.13046214964161024No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5810.12654175115488406No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5710.12436375199559171No Hit
TTTCTGCAGACTATGCTGCGTAAAGAGGTTA5670.1234925523318748No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5540.12066115342479478No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5470.11913655401329015No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5380.11717635476992706No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5310.11565175535842244No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5210.1134737561991301No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC4950.10781095838497007No Hit
ATGGAATCTTCTGCCAAACGTAAAATGGATC4950.10781095838497007No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4880.10628635897346544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)