FastQCFastQC Report
Wed 31 May 2023
EGAF00007963612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493936
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12230.24760292831459949No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT11250.227762301188818No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT10390.21035113860905058No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC9890.2002283696673253No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9290.18808104693725503No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8850.1791730102685368No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC8500.17208707200932913No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7990.1617618476887694No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA7190.145565417382009No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7080.14333840821482946No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6640.13443037154611123No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6530.13220336237893168No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC6360.1287616209387451No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5680.11499465517799876No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5240.10608661850928056No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG5220.10568170775161155No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT5190.10507434161510802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)