FastQCFastQC Report
Wed 31 May 2023
EGAF00007963619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464015
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66661.436591489499262No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA18880.40688339816600755No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17950.38684094264194047No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12620.2719739663588462No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11430.24632824369901835No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC10590.22822538064502226No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8960.19309720590929172No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA8400.181028630539961No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7930.17089964764070129No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7700.16594291132829758No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7630.16443433940713123No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC7510.16184821611370323No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6250.13469392153270907No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT5850.1260735105546157No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5280.11378942491083262No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5180.11163432216630927No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG5070.10926370914733359No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5000.10775513722616727No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG4860.10473799338383458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)