FastQCFastQC Report
Wed 8 May 2024
EGAF00007963686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448986
Total Bases19.7 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT43840.976422427425354No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC42730.9517000530083343No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC38930.8670648973464652No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG27230.6064777075454468No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC21760.4846476282111246No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG14150.3151545927935392No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG13860.30869559407197555No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG12440.2770687727456981No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC11800.2628144307394885No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11320.2521236742348314No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11210.2496737092025141No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9420.20980609640389677No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA9050.20156530493155692No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG8360.18619734245611222No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT8200.18263375695455983No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG7920.17639748232684316No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7670.17082937998066755No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT7540.16793396676065625No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6820.1518978320036705No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6320.14076162731131928No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6120.1363071454343788No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6020.13407990449590856No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5930.13207538765128535No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5890.13118449127589726No Hit
CGGACCGGACGGCATGTCTTTGGCTGGTGCCTGTGCAGGCGGAC5660.1260618371174157No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5640.12561638892972163No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5520.12294369980355735No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5380.11982556248969901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)