FastQCFastQC Report
Wed 8 May 2024
EGAF00007963690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456845
Total Bases20.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT45701.0003392835644476No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC43790.9585307927196314No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC39130.8565268307631692No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG26000.5691208177828366No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC22410.4905383664043603No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG14270.31235977191388764No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG14060.3077630268471801No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG13280.290689402313695No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12220.2674867843579332No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC11950.2615766835578807No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT11410.2497564819577756No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9840.21539034026858125No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8920.19525221902395778No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG8760.19174993706837112No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT8580.1878098698683361No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT8110.17752191662380018No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7680.16810953386816097No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7550.1652639297792468No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG7210.15782158062362509No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6630.14512580853462334No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6510.14249909706793332No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6310.13812124462344996No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6260.1370267815123291No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5830.12761439875668992No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5810.12717661351224158No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5810.12717661351224158No Hit
CGGACCGGACGGCATGTCTTTGGCTGGTGCCTGTGCAGGCGGAC5660.12389322417887905No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA4900.1072573848898423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)