FastQCFastQC Report
Wed 8 May 2024
EGAF00007963695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447185
Total Bases13.8 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70331.5727271710813198No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT23340.5219316390308262No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12600.281762581481937No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11250.2515737334660152No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10950.2448651005735881No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC10050.2247392018963069No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6510.14557733376566745No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6030.13484352113778414No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6010.13439627894495568No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5570.12455695070272929No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5530.12366246631707237No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5480.12254436083500117No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5420.12120263425651576No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5190.11605934903898833No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA4750.10622002079676196No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4550.10174759886847726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)