FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007963700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501613
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT37700.7515754177024918No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT28370.5655754535867292No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26460.5274982905147992No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA24840.49520247680981155No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA22450.4475561837512186No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17300.34488739326931317No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA17080.340501542025426No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT15930.31757550143237917No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15550.3099999401929376No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14740.29385203334044374No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14040.27989705210989346No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG12380.2468038109060172No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC11980.23882953591713132No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10280.20493886721436647No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10180.202945298467145No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG9280.18500317974215183No Hit
GGTGTCAGAAACTTTCGGGACTTCCGGGCTTTTAGGAGCCTCCG9140.18221218349604176No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT8920.17782633225215455No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT8210.16367199414688216No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8170.16287456664799357No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT7540.15031508354049836No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7340.14632794604605542No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7260.14473309104827825No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7200.14353694979994536No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6990.1393504554307803No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT6970.138951741681336No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC6160.12280383482884216No Hit
TGGTGCAGGAGTTTCCGGAGCAGGTGCTTCCGGGATCTGCGGGG6160.12280383482884216No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6080.12120897983106498No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6080.12120897983106498No Hit
GGATGGCGGAGTCAGGCCTTTTTCCTTGGCGTAAGCCTGTGCCG5720.11403213234106771No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5640.11243727734329054No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5400.10765271234995905No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5350.10665592797634829No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5240.10446300235440469No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5190.10346621798079396No Hit
ACGATCGCCGAAGCGGCTGGATTTGGAAGTACCGGTGCCGAAAC5160.10286814735662753No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA5090.1014726492335725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)