FastQCFastQC Report
Wed 8 May 2024
EGAF00007963701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501613
Total Bases15.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39410.7856654432799789No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT38550.7685207520538742No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37800.7535689864497133No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA34980.6973503477780679No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA29750.5930867022983853No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA26590.5300899298861872No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24370.4858327036978706No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA18230.3634275826184728No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG18180.3624307982448621No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17960.35804494700097483No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16970.33830861640348237No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16350.3259484901707093No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15450.3080063714457161No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT12820.2555755133937916No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10680.2129131422032523No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10530.20992278908242012No Hit
CCGAAAGTGTCCGACACCCCGAAAGCCCCGG9310.18560125036631825No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8920.17782633225215455No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8620.17184562601049017No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8600.17144691226104586No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG8450.16845655914021368No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7930.15809000165466205No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7900.1574919310304956No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT7850.15649514665688488No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7760.15470093478438557No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA7550.1505144404152205No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7230.14413502042411183No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7140.1423408085516125No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7020.13994852605494673No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6860.1367588160593924No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6460.12878454107050655No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC6380.12718968607272937No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6070.12100962295634284No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6060.12081026608162068No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5900.11762055608606635No Hit
GACACCCCGCAGGTTCCGGAAGCCCCGAAAT5870.11702248546189992No Hit
AAACTGGCAGGCAAATACACTACCGAGGACG5830.11622505796301133No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5550.11064306547079122No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5520.11004499484662479No Hit
GCCCCGGACACCCCGCAGGTGCCGGAGGCTC5460.10884885359829191No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5320.10605785735218186No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5300.10565914360273757No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5250.10466235922912684No Hit
TTCGGCGGTGGCATCGAAGCACTGGATGGCG5170.10306750423134967No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5150.10266879048190537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)