FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007963703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462860
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74361.606533293004364No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35630.769779198893834No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT35130.7589767964395281No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA32080.6930821414682625No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA27400.5919716544959599No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24030.5191634619539386No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22640.48913278313096836No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16350.3532385602558009No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16280.35172622391219804No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG16110.3480534070777341No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15280.3301214190035864No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15270.3299053709545003No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13380.2890722896772242No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT11680.2523441213325843No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9770.21107894395713608No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8830.1907704273430411No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8610.18601737026314652No Hit
CCGAAAGTGTCCGACACCCCGAAAGCCCCGG8490.18342479367411313No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8430.1821285053795964No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8050.173918679514324No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG8000.1728384392688934No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7690.1661409497472238No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7240.1564187875383485No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT7240.1564187875383485No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7050.1523138746057123No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6960.15036944216393727No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6660.14388800069135374No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6620.14302380849500929No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6520.1408633280041481No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6370.13762260726785636No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5680.12271529188091432No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5620.12141900358639761No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5500.1188264269973642No Hit
TTCGGCGGTGGCATCGAAGCACTGGATGGCG5480.11839433089919198No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5300.11450546601564189No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5250.1134252257702113No Hit
GACACCCCGCAGGTTCCGGAAGCCCCGAAAT5230.11299312967203907No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5200.11234498552478071No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5190.11212893747569459No Hit
AAACTGGCAGGCAAATACACTACCGAGGACG5080.10975240893574731No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5050.10910426478848896No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG4910.10607959210128333No Hit
GCCCCGGACACCCCGCAGGTGCCGGAGGCTC4850.10478330380676662No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4760.10283887136499158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)