FastQCFastQC Report
Wed 8 May 2024
EGAF00007963704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504254
Total Bases22.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT37330.7403015147128233No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT29150.5780816810575623No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26250.5205709820844258No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA24170.4793219290278312No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA21340.4231994193402531No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17180.34070131322706415No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA16530.32781098414687837No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT15690.31115271272017675No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15370.30480670455762376No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT14600.2895366224164806No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13760.272878350989779No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG12730.25245213721656146No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC12580.24947744589036477No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10720.21259127344552547No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10520.20862501834392985No Hit
GGTGTCAGAAACTTTCGGGACTTCCGGGCTTTTAGGAGCCTCCG9560.18958699385627087No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8570.1699540311033725No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8550.16955740559321295No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT8500.16856584181781403No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT8160.16182320814510148No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7870.15607213824778782No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT7700.15270082141143154No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7560.14992444284031461No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT7200.14278518365744247No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7200.14278518365744247No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7120.14119868161680424No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6430.12751510151629933No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC6170.12235896988422501No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA5970.11839271478262939No Hit
TGGTGCAGGAGTTTCCGGAGCAGGTGCTTCCGGGATCTGCGGGG5960.1181944020275496No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC5940.11779777651739005No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5880.11660789998691136No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5830.11561633621151246No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5730.11363320866071465No Hit
GGATGGCGGAGTCAGGCCTTTTTCCTTGGCGTAAGCCTGTGCCG5440.107882138763401No Hit
ACGATCGCCGAAGCGGCTGGATTTGGAAGTACCGGTGCCGAAAC5380.1066922622329223No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG5370.10649394947784252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)