FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007963705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504254
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC39900.7912678927683272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38760.7686602386892321No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT37790.7494239014464933No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA35330.7006389636968671No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA30300.6008876478917371No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25920.514026661166793No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23500.46603497443748587No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG17710.35121188924629254No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17620.3494270744505745No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17390.34486588108373956No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17080.3387181856762663No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16590.329000860677357No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14560.28874337139616146No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT13530.268317157622944No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11000.21814403058775933No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10950.21715246681236045No Hit
CCGAAAGTGTCCGACACCCCGAAAGCCCCGG10070.19970094436533967No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9090.18026629436752115No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8830.17511016273544683No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8600.17054896936861186No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8460.1677725907974949No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG8310.1647978994712982No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA8240.16340971018573971No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7700.15270082141143154No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7700.15270082141143154No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT7390.1465531260039583No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7120.14119868161680424No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6730.13346448416869278No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6720.13326617141361297No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6710.1330678586585332No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC6520.12929991631201737No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6400.12692016325105998No Hit
GACACCCCGCAGGTTCCGGAAGCCCCGAAAT6190.12275559539438458No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6180.12255728263930481No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5910.11720283825215069No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5850.11601296172167203No Hit
AAACTGGCAGGCAAATACACTACCGAGGACG5740.11383152141579442No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5660.11224501937515617No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5630.11165008110991681No Hit
GCCCCGGACACCCCGCAGGTGCCGGAGGCTC5460.10827876427356053No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5460.10827876427356053No Hit
TTCGGCGGTGGCATCGAAGCACTGGATGGCG5440.107882138763401No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5320.1055023857024436No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5300.10510576019228406No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5140.10193275611100755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)