FastQCFastQC Report
Wed 8 May 2024
EGAF00007963707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007963707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471747
Total Bases14.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78401.661907759879766No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC36840.7809270647190125No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT35370.749766294221267No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA33810.7166977214481491No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA27310.5789120015601583No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24200.5129868340445196No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22110.4686834256497656No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16800.356123091402807No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16540.3506116626072874No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG16460.3489158383625121No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16070.3406486951692327No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15340.3251742989356583No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13700.2904099019177652No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT11650.24695440564539892No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10220.21664154727004092No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9880.20943429422974605No Hit
CCGAAAGTGTCCGACACCCCGAAAGCCCCGG8960.1899323154148304No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8340.17678967751782207No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8080.17127824872230243No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7930.16809857826334879No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7880.16703868811036424No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA7760.16449495174320133No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7680.16279912749842607No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT7390.1566517646111157No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6920.146688797173061No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6860.14541692898947953No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6760.14329714868351046No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6630.14054143428575064No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6470.13714978579620007No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6420.13608989564321555No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6120.12973055472530826No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC5860.12421912592978865No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA5680.12040352137904428No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5580.11828374107307518No Hit
AAACTGGCAGGCAAATACACTACCGAGGACG5570.11807176304247828No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5410.11468011455292773No Hit
GACACCCCGCAGGTTCCGGAAGCCCCGAAAT5280.11192440015516793No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5280.11192440015516793No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5120.10853275166561736No Hit
GCCCCGGACACCCCGCAGGTGCCGGAGGCTC5020.10641297135964829No Hit
TTCGGCGGTGGCATCGAAGCACTGGATGGCG4980.10556505923726064No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG4900.10386923499248538No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4890.10365725696188847No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4850.10280934483950083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)