FastQCFastQC Report
Wed 31 May 2023
EGAF00007964611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007964611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48938841
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATACG1220864324.9467350483433Illumina Single End PCR Primer 1 (100% over 8bp)
TGTGCCGT1204633424.615078236119242No Hit
CCACAGAA1193824724.39421685527861No Hit
GACTGTTC1143339123.362610896322618No Hit
GACTGTTA707230.1445130259623435No Hit
GCCTGTTC532260.10876023811025684No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)