FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007966581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007966581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459860
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG31280.6802070195276824No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG25740.5597355716957335No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG24160.5253772887400514No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG24050.5229852563823773No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA20640.44883225329448095No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG20540.44665767842386817No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG19790.4303483668942722No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19250.41860566259296306No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18820.4092549906493281No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16800.3653285782629496No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG16260.3535858739616405No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT15790.34336537206976037No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG15330.3333623276649415No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG14700.3196625059800809No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14220.30922454660113946No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13870.3016135345539947No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA13830.30074370460574956No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC13740.29878658722219803No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG13090.28465185056321485No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12540.2726916887748445No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG12300.2674727090853738No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG11780.2561649197581873No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11680.2539903448875745No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT11650.2533379724263906No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG10890.23681120340973338No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10820.23528900100030442No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG10740.2335493411038142No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10380.22572087156960816No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10230.22245900926368892No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10040.21832731700952462No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT9700.21093376244944112No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9400.20441003783760275No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG9320.2026703779411125No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT9280.2018005479928674No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG9180.19962597312225458No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG9100.19788631322576436No Hit
ACGAGTCTCGATCATAGATTCATCAGAGGTGTTGGAGCTCGCAC9090.1976688557387031No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG8600.1870134388727004No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG8580.18657852389857782No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT8540.1857086939503327No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA8370.18201191667029096No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT7630.16592006262775627No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC7520.1635280302700822No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT7470.1624407428347758No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG7270.1580915930935502No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT7080.1539599008393859No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT6910.15026312355934413No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG6830.1485234636628539No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6700.14569651633105726No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6700.14569651633105726No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6630.14417431392162833No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6250.1359109294132997No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG6160.13395381202974818No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5940.12916974731440004No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5720.12438568259905189No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5560.12090636280607141No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5450.11851433044839735No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG5410.11764450050015221No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5360.11655721306484583No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5270.1146000956812943No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4870.10590179619884312No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4670.10155264645761754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)