FastQCFastQC Report
Wed 8 May 2024
EGAF00007966582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007966582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459860
Total Bases14.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45180.9824729265428609No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG34290.7456617231331275No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA31830.6921671813160527No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC28800.626277562736485No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27670.6017048666985605No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG27280.5932240247031705No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG26090.5673465837428783No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG24230.5268994911494803No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23500.5110250945940069No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC23250.5055886574174748No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG20080.43665463401904925No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG19860.43187056930370116No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC19030.4138215978776149No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC17090.3716348453877267No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG16780.364893663288827No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA14780.3214021658765711No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14770.32118470838950985No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT14160.3079198016787718No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG13530.2942199799939112No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13400.2913930326621145No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG12890.2803027008219893No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12620.27443134867133473No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG12490.2716044013395381No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC12290.26725525159831254No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG11570.2515983125299004No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11390.24768407776279738No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11350.24681424781455227No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11270.245074587918062No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG11070.24072543817683645No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG10880.23659374592267213No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG10660.23180968120732398No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10210.2220240942895664No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG9780.2126734223459314No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG9530.20723698516939937No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9470.20593224024703172No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT9220.2004958030704997No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8940.1944069934327839No Hit
GACTCCGTTTCTCGTGCGCTGACTCAGGCAC8870.19288479102335493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8830.19201496107510982No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA8350.1815770016961684No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8060.17527073457139128No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8050.17505327708433002No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7960.17309615970077852No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7760.1687470099595529No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7460.16222328534771452No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7020.15265515591701823No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6890.14982820858522158No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC6750.14678380376636366No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6450.1402600791545253No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC6360.1383029617709738No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6240.13569347192623843No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG6040.13134432218501285No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5930.12895228982733875No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5680.12351585265080677No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5500.11960161788370374No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5460.11873178793545862No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5440.11829687296133606No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5340.11612229809072326No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5240.11394772322011047No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC5160.11220806332362024No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC4800.10437959378941417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)