FastQCFastQC Report
Wed 8 May 2024
EGAF00007966607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007966607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540583
Total Bases23.7 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT32920.6089721652364207No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC31910.5902886328278913No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT27650.5114848228671638No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC26310.486696769968719No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20510.379405197721719No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG17830.3298290919248293No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16570.3065209227815155No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15690.2902422014750741No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15650.28950225959750864No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15240.28191785535246205No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC15030.2780331604952431No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13610.2517652238416672No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12810.2369663862903569No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12630.23363664784131205No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12370.2288270256371362No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11590.2143981590246086No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9720.17980587624842068No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9370.1733313848197224No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9000.16648692245224136No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8930.16519202416650172No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8930.16519202416650172No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA8850.1637121404113707No Hit
GCAACCGGTGCGTTCTGCCAGGGACGGGTCCAGGTCATCAGACA8450.15631272163571552No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA8170.1511331284927569No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7510.13892408751292587No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT7470.13818414563536036No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG7320.13540936359448966No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7180.13281956702301034No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC7020.12985979951274826No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG6490.12005556963500517No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6350.11746577306352586No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6300.11654084571656896No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6230.11524594743082932No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6000.11099128163482759No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5820.10766154318578276No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5820.10766154318578276No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5750.1063666449000431No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5430.10044710987951895No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5420.10026212441012758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)