FastQCFastQC Report
Wed 8 May 2024
EGAF00007966608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007966608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540583
Total Bases16.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA71861.3293055830464517No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA36760.6800065854827103No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA34700.6418995787880862No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27730.5129647066222949No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22000.4069680326610345No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20710.38310490710954653No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19080.3529522755987517No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17880.3307540192717862No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17190.317990021883781No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16210.29986144588342584No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15250.28210284082185344No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13990.25879467167853965No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13600.25158023837227583No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13440.2486204708620138No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13050.24140603755575No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10740.19867439412634136No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9860.18239567281990002No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA9250.17111155918702586No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9160.16944668996250342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9140.16907671902372068No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8940.1653770096358931No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8810.16297219853380515No Hit
AAGCTGAAGGAAGCGGAAGCACGTGCCGAGT8580.15871753273780345No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC8410.15557277975815No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8110.1500232156764086No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7910.14632350628858104No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC7370.13633429094144653No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7290.1348544071863155No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA7270.13448443624753276No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7050.13041475592092241No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC6990.12930484310457413No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6660.12320032261465862No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6480.1198705841656138No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5980.11062131069604483No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5930.10969638334908792No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5910.10932641241030516No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5850.10821649959395689No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5800.107291572247No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5600.1035918628591724No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5460.1010020662876931No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5430.10044710987951895No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5410.1000771389407362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)