FastQCFastQC Report
Wed 8 May 2024
EGAF00007966610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007966610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517834
Total Bases16 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA68391.3206935040959071No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42780.8261334713440988No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35400.6836167574937142No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31670.6115859522549697No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG25550.4934013602814802No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC20270.3914381828925872No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20020.38661038093288586No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17370.33543568016005126No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17020.32867675741646935No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG16440.31747625686996217No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14940.28850944511175397No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14140.2730604788407096No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14090.27209491844876926No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12630.2439005550041133No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12200.23559673563342695No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11890.2296102612033972No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10800.20856104465909925No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA8960.17302842223569714No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8960.17302842223569714No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8450.16317970623790637No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8410.16240725792435415No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8180.15796568012142886No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC7640.1475376278884739No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7590.14657206749653365No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7280.14058559306650392No Hit
AAGCTGAAGGAAGCGGAAGCACGTGCCGAGT7280.14058559306650392No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6990.13498534279325036No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6780.1309299891471012No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC6340.12243305769802679No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6220.12011571275737012No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6210.11992260067898207No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC6160.11895704028704178No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6150.11876392820865374No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6070.1172190315815493No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5600.10814276389731071No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5450.10524608272148991No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5410.10447363440793768No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5390.10408741025116157No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5380.10389429817277351No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5330.10292873778083324No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5280.10196317738889298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)